Multicolor Timelapse Luminescence Microscopy: Optimizing Luciferases to Track Fast Gene Dynamics in Single Yeast Cells
نویسندگان
چکیده
منابع مشابه
Measuring fast gene dynamics in single cells with time-lapse luminescence microscopy
Time-lapse fluorescence microscopy is an important tool for measuring in vivo gene dynamics in single cells. However, fluorescent proteins are limited by slow chromophore maturation times and the cellular autofluorescence or phototoxicity that arises from light excitation. An alternative is luciferase, an enzyme that emits photons and is active upon folding. The photon flux per luciferase is si...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2014
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2013.11.2109